Research in the Marty Lab is centered on characterization of membrane proteins and antimicrobial peptides by mass spectrometry. Our lab works at the interface of chemistry and biochemistry, combining cutting-edge analytical methodologies with biotechnology.
Congratulations for a string of recent awards to people in the Marty Lab. Grad student James Keener received the Carl S. Marvel Fellowship, the top fellowship in the Department of Chemistry and Biochemistry at UA. Grad student Julia Townsend was awarded the Early Career Research Award, the NIH T32 Biological Chemistry Program (BCP) Fellowship, and the James D. Steele Fellowship. Prof. Marty was awarded the Galileo Circle Curie Award from the College of Science at UA. Congrats to all!
New Paper on Native MS of Rhodopsin
Congratulations to Carolanne and all the collaborators in the Marty and Brown labs on our latest paper in the International Journal of Mass Spectrometry using native MS to study zinc and lipid binding to rhodopsin!
Norris, C.E.; Keener, J.E.; Perera, S. M. D. C.; Weerasinghe, N.; Fried, S.D.E.; Resager, W.C.; Rohrbough, J.G.; Brown, M.F.; Marty, M.T. “Native Mass Spectrometry Reveals the Simultaneous Binding of Lipids and Zinc to Rhodopsin” Int. J. Mass Spectrom. 2020. DOI: 10.1016/j.ijms.2020.116477.
Featured Review in Analytical Chemistry
Congratulations to James and Zhi on their publication of their review on Native MS of membrane proteins in the Fundamental and Applied Reviews in Analytical Chemistry 2021 special issue!
Keener, J.E.; Zhang, G.; Marty, M.T. “Native Mass Spectrometry of Membrane Proteins” Anal. Chem. 2020. DOI: 10.1021/acs.analchem.0c04342.
New Young Scientist Perspective on Nanodiscs and Native MS
Prof. Marty recently published a Young Scientist Perspective in the International Journal of Mass Spectrometry on using nanodiscs with native MS. This article is a great place to start for those interested in learning more about our research.
Marty, M.T. “Nanodiscs and Mass Spectrometry: Making Membranes Fly” Int. J. Mass Spectrom. 2020. DOI: 10.1016/j.ijms.2020.116436.
Using Native MS to Study New Drugs for SARS-CoV-2
PhD student Julia Townsend has been hard at work on a collaborative project with Prof. Jun Wang in the UA College of Pharmacy to look at new drugs bound to the SARS-CoV-2 Main Protease with native MS. The first paper was just published in Cell Research.
Ma, C.; Sacco, M.D.; Hurst, B.; Townsend, J.A.; Hu, Y.; Szeto, T.; Zhang, X.; Tarbet, B.; Marty, M.T.; Chen, Yu; Wang, J. “Boceprevir, GC-376, and calpain inhibitors II, XII inhibit SARS-CoV-2 viral replication by targeting the viral main protease” Cell Research. 2020, DOI: 10.1038/s41422-020-0356-z.
Building on this initial work, we recently published a paper in ACS Pharmacology and Translational Science exploring the specificity of various inhibitors:
Ma, C.; Hu, Y.; Townsend, J.A.; Lagarias, P.I.; Marty, M.T.; Kolocouris, A.; Wang, J. “Ebselen, Disulfiram, Carmofur, PX-12, Tideglusib, and Shikonin Are Nonspecific Promiscuous SARS-CoV-2 Main Protease Inhibitors”. ACS Pharmacol. Transl. Sci. 2020. DOI: 10.1021/acsptsci.1020c00130.
Finally, a paper was recently published using similar methods on novel Mpro inhibitors in Science Advances:
Sacco, M.D.; Ma, C.; Lagarias, P.; Gao, A.; Townsend, J.A.; Meng, X.; Dube, P.; Zhang, X.; Hu, Y.; Kitamura, N.; Hurst, B.; Tarbet, B.; Marty. M.T.; Kolocouris, A.; Xiang, Y.; Chen, Y.; Wang, J. “Structure and inhibition of the SARS-CoV-2 main protease reveals strategy for developing dual inhibitors against Mpro and cathepsin L”. Sci. Adv. 2020. DOI: 10.1126/sciadv.abe0751.
New Article in Biochemistry
Congrats to Larry on his new paper exploring a wide range of antimicrobial peptides in nanodiscs with native MS!
Walker, L.R.; Marty, M.T. “Revealing the Specificity of a Range of Antimicrobial Peptides in Lipid Nanodiscs by Native Mass Spectrometry” Biochemistry. 2020, DOI: 10.1021/acs.biochem.0c00335
New Letter in Analytical Chemistry
Congrats to Zhi and James on their excellent new paper describing native MS and LX-MS for measuring membrane protein-lipid interactions!
Zhang, G.; Keener, J.E.; Marty, M.T. “Measuring Remodeling of the Lipid Environment Surrounding Membrane Proteins with Lipid Exchange and Native Mass Spectrometry.” Anal. Chem. 2020, DOI: 10.1021/acs.analchem.1020c00786.
New UniDec Tutorial Videos
Funded by NSF, we have released our first videos in a series of UniDec tutorial videos.
In Part 1, PhD student, Deseree Reid, talks about importing data from a variety of formats into UniDec: https://youtu.be/y6geXLm9CzI
In Part 2, PhD student, Marius Kostelic, talks about the basics of data processing: https://youtu.be/Dp9eNR6sfmU.
In Part 3, Marius talks about advanced data processing: https://youtu.be/MxR3KgWUtGc.
In Part 4, Marius talks about introductory deconvolution settings and results: https://youtu.be/vFZpJuVhHT0.
In Part 5, Marius goes over how UniDec works and Quick Controls: https://youtu.be/j7b8yU1Sn44.
Two New Articles
Enjoy these two new articles. One is on scoring ESI deconvolution and the other is a review article with the excellent Prof. Michael Landreh on using native MS to study peripheral membrane protein interactions.
Marty, M.T. “A Universal Score for Deconvolution of Intact Protein and Native Electrospray Mass Spectra.” Anal. Chem. 2020, DOI: 10.1021/acs.analchem.1029b05272.
Sahin, C.; Reid, D.J.; Marty, M.T.; Landreh, M. “Scratching the surface: Native mass spectrometry of peripheral membrane protein complexes.” Biochem. Soc. Trans. 2020, DOI: 10.1042/bst20190787.
New Article in Analytical Chemistry
Congratulations to Julia, James, and our collaborators in the Prell lab for their new article in Analytical Chemistry on using novel charge reducing reagents to improve the stability of complexes for native MS.
Townsend, J.A.; Keener, J.E.; Miller, Z.; Prell, J.S.; Marty, M.T. "Imidazole Derivatives Improve Charge Reduction and Stabilization for Native Mass Spectrometry." Anal. Chem. 2019, DOI: 10.1021/acs.analchem.1029b04263.
Prof. Marty on NPR
Prof. Marty sat down with Arizona Public Media to discuss their new work with antimicrobial peptides with Prof. Leslie Tolbert. Listen to the interview here: https://radio.azpm.org/p/radio-azscience/2019/8/23/157065-episode-196-molecules-and-the-battle-against-bacteria/.
National Science Foundation CAREER Award
Congratulations to Prof. Marty on receiving an NSF CAREER award! This will fund new work in the lab on using native MS to study interactions of membrane active compounds such as antimicrobial peptides with lipid nanodiscs. It also supports ongoing development of UniDec. New tutorials for UniDec will be coming soon. Finally, it also supports development of the ASEMS-V program to engage Veterans in STEM Research in collaboration with the University of Arizona ASEMS program and the VETS center. Stay tuned...
New Article in Analytical Chemistry
Congrats to Larry, Elaine, Julia, and Will on their new article in Analytical Chemistry on using native MS to study antimicrobial peptide complex formation and lipid interactions in nanodiscs. It launches a new direction in the Marty lab on studying antimicrobial peptides in intact nanodiscs by native MS.
Walker, L.R.; Marzluff, E.M.; Townsend, J.A.; Resager, W.C.; Marty, M.T. "Native Mass Spectrometry of Antimicrobial Peptides in Lipid Nanodiscs Elucidates Complex Assembly." Anal. Chem. 2019, DOI: 10.1021/acs.analchem.1029b02261.
New Article in the Journal of the American Society for Mass Spectrometry
Congrats to Marius, Alex, Deseree and Jibriel on their new article in JASMS. This work focuses on native MS analysis of nanodiscs with a wider range of lipids, including glycolipid GM1, cardiolipin, and sterols. It also include our first results with Saposin A nanoparticles. Great work!
Kostelic, M.M.; Ryan, A.M.; Reid, D.J.; Noun, J.M.; Marty, M.T. Expanding the Types of Lipids Amenable to Native Mass Spectrometry of Lipoprotein Complexes. J. Am. Soc. Mass Spectrom. 2019, DOI: 10.1007/s13361-019-02174-x.
New Article in the Journal of the American Chemical Society
Congrats to James, Dane, Guozhi, Ciara, and Deseree on the publication of their paper in JACS! This work demonstrated the first native MS measurement of membrane protein oligomeric state within a lipid bilayer environment. Deseree did an awesome job sketching the concept below. Thanks also to collaborators in the Pemberton and Prell labs. Well done team!
Keener, J.E.; Zambrano, D.E.; Zhang, G.; Zak, C.K.; Reid, D.J.; Deodhar, B.S.; Pemberton, J.E.; Prell, J.S.; Marty, M.T. Chemical additives enable native mass spectrometry measurement of membrane protein oligomeric state within intact nanodiscs. J. Am. Chem. Soc. 2019, 141, 1054-1061.
National Institutes of Health MIRA Award
Congratulations to Prof. Marty on being awarded the National Institutes of Health/National Institute of General Medical Sciences Maximizing Investigator's Research Award (MIRA) R35 GM128624! This grant will support the lab efforts to employ native mass spectrometry and nanodiscs to understanding membrane protein-lipid interactions.
Congrats to Dane and Jessica on Graduating
Congratulations to Dane Zambrano and Jessica Diesing on their graduation and successful completion of their undergraduate theses. Jessica was also recently awarded the CBC Outstanding Peer Mentor Award and CBC Student of the Month. Congrats!
American Society for Mass Spectrometry Research Award
Congratulations to Prof. Marty on being awarded one of the 2018 ASMS Research Awards! Thanks to Thermo Fisher for sponsoring the award.
New Article in Journal of the American Society for Mass Spectrometry
Check out our new article on MetaUniDec, software for high throughput deconvolution of native mass spectra:
Reid, D. J.; Diesing, J. M.; Miller, M. A.; Perry, S. M.; Wales, J. A.; Montfort, W. R.; Marty, M. T. MetaUniDec: High-Throughput Deconvolution of Native Mass Spectra. J. Am. Soc. Mass. Spectrom. 2018, DOI: 10.1007/s13361-13018-11951-13369.
The software can be downloaded from links in the software tab. Happy deconvolving!
Congrats to Dane Zambrano
Undergrad, Dane Zambrano, has been with the Marty Lab since Day 1. She is graduating soon and has garnered some of the top awards from the CBC department, including the Excellence in Undergraduate Research Award. Congrats to Dane and good luck in grad school at UW Seattle!
New Article in Analytical Chemistry
Check out our new article describing engineering of nanodiscs for native mass spectrometry.
Reid, D.J.; Keener, J.E.; Wheeler, A.P.; Zambrano, D.E.; Diesing, J.M.; Reinhardt-Szyba, M.; Makarov, A.A.; Marty, M.T. "Engineering Nanodisc Scaffold Proteins for Native Mass Spectrometry" Anal. Chem. 2017, DOI: 10.1021/acs.analchem.7b03569
Plasmids used in this study can be acquired from Addgene: http://www.addgene.org/Michael_Marty/
Bisgrove Scholar Award
New Review Article
A review of membrane mimetics for mass spectrometry was recently published in Accounts of Chemical Research. For those interested in learning more about our research, this is a great place to start.
Marty, M.T.; Hoi, K.K.; Robinson, C.V. "Interfacing Membrane Mimetics with Mass Spectrometry" Acc. Chem. Res. 2016, DOI: 10.1021/acs.accounts.6b00379