Welcome to the Marty Lab

Research in the Marty Lab is centered on characterization of membrane proteins and antimicrobial peptides by mass spectrometry. Our lab works at the interface of chemistry and biochemistry, combining cutting-edge analytical methodologies with biotechnology.


New Paper in JASMS

Interested in learning more about the basics of native MS statistics? Check out this primer from Prof. Marty:

Marty, M.T. "Fundamentals: How Do We Calculate Mass, Error, and Uncertainty in Native Mass Spectrometry?J. Am. Soc. Mass. Spectr. 2022, DOI: 10.1021/jasms.1022c00218.

TOC for Marty JASMS 2022

New Paper in Analytical Chemistry

Congrats to Marius and the team on the latest paper in Analytical Chemistry! He used variable temperature ionization with charge detection-mass spectrometry to characterize the stability and dissociation of AAV capsids. Enjoy!

Kostelic, M.M.; Ryan, J.P.; Brown, L.S.; Jackson, T.W.; Hsieh, C.C.; Zak, C.K.; Sanders, H.M.; Liu, Y.; Chen, V.S.; Byrne, M.; Aspinwall, C.A.; Baker, E.S.; Marty, M.T.Stability and Dissociation of Adeno-Associated Viral Capsids by Variable Temperature-Charge Detection-Mass Spectrometry.” Anal. Chem. 2022, DOI: 10.1021/acs.analchem.1022c02378.

TOC Kostelic 2022 Anal Chem


Want to learn more about how to use UniDec?

Check out this protocol that Marius wrote during the pandemic:

Kostelic, M.M.Marty, M.T.Deconvolving Native and Intact Protein Mass Spectra with UniDec. In Proteoform Identification: Methods and Protocols; Sun, L., Liu, X., Eds.; Springer US: New York, NY, 2022; pp 159-180.

It's also on ChemRxiv for those who don't have access. Enjoy!


New Paper in Analytical Chemistry

Congrats to James and Hiruni on a long awaited paper in Analytical Chemistry! They explored how nanodisc native MS can be used to probe membrane protein-lipid interactions. Check it out!

Keener, J.E.Jayasekera, H.S.Marty, M.T. “Investigating the Lipid Selectivity of Membrane Proteins in Heterogeneous Nanodiscs.” Anal. Chem. 2022, DOI:10.1021/acs.analchem.2c01488.

TOC Keener 2022 Anal Chem

New Paper in Biochemistry

Congrats to Henry on his first lead author paper from the Marty Lab in Biochemistry. Henry looked at how lipid properties and EGCG (a molecule present in green tea) affect alpha-synuclein interactions with nanodiscs. Alpha-synuclein is a protein important in Parkinson's Disease, and Henry's data revealed that EGCG blocked membrane disruption but not association. Congrats!

Sanders, H.M.; Kostelic, M.M.; Zak, C.K.; Marty, M.T. “Lipids and Small Molecules Affect α-synuclein Association and Disruption of Nanodiscs.” Biochemistry 2022, DOI: 10.1021/acs.biochem.2c00160.

TOC Sanders 2022 Biochemistry


New Paper in JASMS

Congrats to Marius on his latest paper in JASMS, which detailed how PEG-coated nESI tips can be used to improve sensitivity for native MS and CD-MS!

Kostelic, M.M.; Hsieh, C.C.; Sanders, H.M.; Zak, C.K.; Ryan, J.P.; Baker, E.S.; Aspinwall, C.A.; Marty, M.T. Surface Modified Nano-Electrospray Needles Improve Sensitivity for Native Mass Spectrometry.” J. Am. Soc. Mass Spectrom. 2022, DOI: 10.1021/jasms.2c00087

TOC Kostelic 2022 JASMS

Congrats to Ciara!

...for winning the CBC Excellence in Research Award, the top research prize for undergraduates in our department! Also, we congratulate her on getting in the UA College of Medicine!

Congrats to Prof. Marty!

...for receiving the ACS Division of Analytical Chemistry Arthur F. Findeis Award for Achievements by a Young Analytical Scientist!

New Paper on How Lipid Tails Affect AMP Incorporation and Activity

Congrats to Larry on his latest paper on using nanodisc native MS and activity assays to determine how lipid tails modulate AMP incorporation and disruption of membranes!

Walker, L.R.; Marty, M.T.Lipid Tails Modulate Antimicrobial Peptide Membrane Incorporation and Activity.Biochim. Biophys. Acta Biomembr. 2022, DOI: 10.1016/j.bbamem.2022.183870.


New Paper on FPOP of AMPs in Nanodiscs

Congrats to Deseree, James, and Marius on their recent paper in JASMS on FPOP of antimicrobial peptides in nanodiscs!

Reid, D.J.; Rohrbough, J.G.; Kostelic, M.M.; Marty, M.T.Investigating Antimicrobial Peptide-Membrane Interactions Using Fast Photochemical Oxidation of Peptides in Nanodiscs.” J. Am. Soc. Mass Spectrom. 2021, DOI: 10.1021/jasms.1021c00252.

FPOP of AMPs in Nanodiscs

New Paper on Influenza A M2 Oligomerization

Congrats to Julia and collaborators for their recent publication on oligomerization of influenza A M2 in different environments! Some controversial but interesting results! Looking forward to seeing more viroporin research soon.

Townsend, J.A.; Sanders, H.M.; Rolland, A.D.; Park, C.K.; Horton, N.C.; Prell, J.S.; Wang, J; Marty, M.T.Influenza A M2 Channel Oligomerization is Sensitive to its Chemical Environment.” Anal Chem. 2021, DOI: 10.1021/acs.analchem.1c04660.

AM2 Oligomerization

New Paper on UniDecCD

Congrats to Marius and the team for publication of the newest UniDec paper! This one describes UniDecCD, the first deconvolution software for charge detection-mass spectrometry data. As always, it's free to download under the software tab. Enjoy!

Kostelic, M.M.; Zak, C.K.; Liu, Y.; Chen, V.; Wu, Z.; Sivinski, J.; Chapman, E.; Marty, M.T.UniDecCD: Deconvolution of Charge Detection-Mass Spectrometry Data.” Anal. Chem. 2021, DOI:10.1021/acs.analchem.1c03181

TOC for Kostelic et al

New Paper in Nature

Congrats to James Keener on publication of his collaborative work in Nature! James used native MS (both from micelles and nanodiscs) to identify an unexpected lipid bound to MFSD2A, the key transporter of omega-3 fatty acids in the blood-brain barrier. Thanks to Rosie Carter and Filippo Mancia for bring us into this exciting collaboration!

Cater, R.J.; Chua, G.L.; Erramilli, S.K.; Keener, J.E.; Choy, B.C.; Tokarz, P.; Chin, C.F.; Quek, D.Q. Y.; Kloss, B.; Pepe, J.G.; Parisi, G.; Wong, B.H.; Clarke, O.B.; Marty, M.T.; Kossiakoff, A.A.; Khelashvili, G.; Silver, D.L.; Mancia, F. Structural basis of omega-3 fatty acid transport across the blood–brain barrier. Nature 2021, DOI: 10.1038/s41586-41021-03650-41589.

ASMS Ron Hites Award

Congrats to Prof. Marty, Marius, and the rest of the team for winning the ASMS Ron Hites Award for an exemplary paper published in the Journal of the American Society for Mass Spectrometry!


New Paper on Native MS of Mixed Lipid Nanodiscs

Congrats to Marius, Ciara, and Hiruni on their excellent paper in Analytical Chemistry describing how to make mixed lipid nanodiscs that model natural membranes yet work for native MS!

Kostelic, M.M.; Zak, C.K.; Jayasekera, H.S.; Marty, M.T. Assembly of Model Membrane Nanodiscs for Native Mass SpectrometryAnal. Chem. 2021, DOI: 10.1021/acs.analchem.1021c00735.

TOC Kostelic 2021

Recent Awards!

Congratulations for a string of recent awards to people in the Marty Lab. Grad student James Keener received the Carl S. Marvel Fellowship, the top fellowship in the Department of Chemistry and Biochemistry at UA. Grad student Julia Townsend was awarded the Early Career Research Award, the NIH T32 Biological Chemistry Program (BCP) Fellowship, and the James D. Steele Fellowship. Prof. Marty was awarded the Galileo Circle Curie Award from the College of Science at UA. Congrats to all!

New Paper on Native MS of Rhodopsin

Congratulations to Carolanne and all the collaborators in the Marty and Brown labs on our latest paper in the International Journal of Mass Spectrometry using native MS to study zinc and lipid binding to rhodopsin!

Norris, C.E.; Keener, J.E.; Perera, S. M. D. C.; Weerasinghe, N.; Fried, S.D.E.; Resager, W.C.; Rohrbough, J.G.; Brown, M.F.; Marty, M.T.Native Mass Spectrometry Reveals the Simultaneous Binding of Lipids and Zinc to Rhodopsin Int. J. Mass Spectrom. 2020. DOI: 10.1016/j.ijms.2020.116477.

TOC Figure Norris IJMS

Featured Review in Analytical Chemistry

Congratulations to James and Zhi on their publication of their review on Native MS of membrane proteins in the Fundamental and Applied Reviews in Analytical Chemistry 2021 special issue!

Keener, J.E.; Zhang, G.; Marty, M.T.Native Mass Spectrometry of Membrane ProteinsAnal. Chem. 2020. DOI: 10.1021/acs.analchem.0c04342.

TOC for Keener et al.

New Young Scientist Perspective on Nanodiscs and Native MS

Prof. Marty recently published a Young Scientist Perspective in the International Journal of Mass Spectrometry on using nanodiscs with native MS. This article is a great place to start for those interested in learning more about our research. 

Marty, M.T.Nanodiscs and Mass Spectrometry: Making Membranes FlyInt. J. Mass Spectrom. 2020. DOI: 10.1016/j.ijms.2020.116436.

TOC Figure for IJMS


Using Native MS to Study New Drugs for SARS-CoV-2

PhD student Julia Townsend has been hard at work on a collaborative project with Prof. Jun Wang in the UA College of Pharmacy to look at new drugs bound to the SARS-CoV-2 Main Protease with native MS. The first paper was just published in Cell Research.

Ma, C.; Sacco, M.D.; Hurst, B.; Townsend, J.A.; Hu, Y.; Szeto, T.; Zhang, X.; Tarbet, B.; Marty, M.T.; Chen, Yu; Wang, J. “Boceprevir, GC-376, and calpain inhibitors II, XII inhibit SARS-CoV-2 viral replication by targeting the viral main proteaseCell Research. 2020, DOI: 10.1038/s41422-020-0356-z.

Building on this initial work, we recently published a paper in ACS Pharmacology and Translational Science exploring the specificity of various inhibitors:

Ma, C.; Hu, Y.; Townsend, J.A.; Lagarias, P.I.; Marty, M.T.; Kolocouris, A.; Wang, J. “Ebselen, Disulfiram, Carmofur, PX-12, Tideglusib, and Shikonin Are Nonspecific Promiscuous SARS-CoV-2 Main Protease Inhibitors”. ACS Pharmacol. Transl. Sci. 2020. DOI: 10.1021/acsptsci.1020c00130.

Finally, a paper was recently published using similar methods on novel Mpro inhibitors in Science Advances:

Sacco, M.D.; Ma, C.; Lagarias, P.; Gao, A.; Townsend, J.A.; Meng, X.; Dube, P.; Zhang, X.; Hu, Y.; Kitamura, N.; Hurst, B.; Tarbet, B.; Marty. M.T.; Kolocouris, A.; Xiang, Y.; Chen, Y.; Wang, J. “Structure and inhibition of the SARS-CoV-2 main protease reveals strategy for developing dual inhibitors against Mpro and cathepsin L”. Sci. Adv. 2020. DOI: 10.1126/sciadv.abe0751.

New Article in Biochemistry

Congrats to Larry on his new paper exploring a wide range of antimicrobial peptides in nanodiscs with native MS!

Walker, L.R.; Marty, M.T.Revealing the Specificity of a Range of Antimicrobial Peptides in Lipid Nanodiscs by Native Mass SpectrometryBiochemistry. 2020, DOI: 10.1021/acs.biochem.0c00335

Peptide Nanodisc Assembly

New Letter in Analytical Chemistry

Congrats to Zhi and James on their excellent new paper describing native MS and LX-MS for measuring membrane protein-lipid interactions!

Zhang, G.Keener, J.E.Marty, M.T. “Measuring Remodeling of the Lipid Environment Surrounding Membrane Proteins with Lipid Exchange and Native Mass Spectrometry.” Anal. Chem. 2020, DOI: 10.1021/acs.analchem.1020c00786.

LXMS and Nanodisc Native MS

New UniDec Tutorial Videos

Funded by NSF, we have released our first videos in a series of UniDec tutorial videos.

In Part 1, PhD student, Deseree Reid, talks about importing data from a variety of formats into UniDec: https://youtu.be/y6geXLm9CzI

In Part 2, PhD student, Marius Kostelic, talks about the basics of data processing: https://youtu.be/Dp9eNR6sfmU

In Part 3, Marius talks about advanced data processing: https://youtu.be/MxR3KgWUtGc

In Part 4, Marius talks about introductory deconvolution settings and results: https://youtu.be/vFZpJuVhHT0.

In Part 5, Marius goes over how UniDec works and Quick Controls: https://youtu.be/j7b8yU1Sn44.

Two New Articles

Enjoy these two new articles. One is on scoring ESI deconvolution and the other is a review article with the excellent Prof. Michael Landreh on using native MS to study peripheral membrane protein interactions.

Marty, M.T. “A Universal Score for Deconvolution of Intact Protein and Native Electrospray Mass Spectra.” Anal. Chem. 2020, DOI: 10.1021/acs.analchem.1029b05272.

Sahin, C.; Reid, D.J.Marty, M.T.; Landreh, M. “Scratching the surface: Native mass spectrometry of peripheral membrane protein complexes.” Biochem. Soc. Trans. 2020, DOI: 10.1042/bst20190787.

New Article in Analytical Chemistry

Congratulations to Julia, James, and our collaborators in the Prell lab for their new article in Analytical Chemistry on using novel charge reducing reagents to improve the stability of complexes for native MS.

Townsend, J.A.Keener, J.E.; Miller, Z.; Prell, J.S.; Marty, M.T. "Imidazole Derivatives Improve Charge Reduction and Stabilization for Native Mass Spectrometry." Anal. Chem. 2019, DOI: 10.1021/acs.analchem.1029b04263.

Imidazole Derivatives

Prof. Marty on NPR

Prof. Marty sat down with Arizona Public Media to discuss their new work with antimicrobial peptides with Prof. Leslie Tolbert. Listen to the interview here: https://radio.azpm.org/p/radio-azscience/2019/8/23/157065-episode-196-molecules-and-the-battle-against-bacteria/

National Science Foundation CAREER Award

Congratulations to Prof. Marty on receiving an NSF CAREER award! This will fund new work in the lab on using native MS to study interactions of membrane active compounds such as antimicrobial peptides with lipid nanodiscs. It also supports ongoing development of UniDec. New tutorials for UniDec will be coming soon. Finally, it also supports development of the ASEMS-V program to engage Veterans in STEM Research in collaboration with the University of Arizona ASEMS program and the VETS center. Stay tuned...

New Article in Analytical Chemistry

Congrats to Larry, Elaine, Julia, and Will on their new article in Analytical Chemistry on using native MS to study antimicrobial peptide complex formation and lipid interactions in nanodiscs. It launches a new direction in the Marty lab on studying antimicrobial peptides in intact nanodiscs by native MS. 

Walker, L.R.; Marzluff, E.M.; Townsend, J.A.; Resager, W.C.; Marty, M.T. "Native Mass Spectrometry of Antimicrobial Peptides in Lipid Nanodiscs Elucidates Complex Assembly." Anal. Chem. 2019, ​​​​​​DOI: 10.1021/acs.analchem.1029b02261.

New Article in the Journal of the American Society for Mass Spectrometry

Congrats to Marius, Alex, Deseree and Jibriel on their new article in JASMS. This work focuses on native MS analysis of nanodiscs with a wider range of lipids, including glycolipid GM1, cardiolipin, and sterols. It also include our first results with Saposin A nanoparticles. Great work!

Kostelic, M.M.; Ryan, A.M.; Reid, D.J.; Noun, J.M.; Marty, M.T. Expanding the Types of Lipids Amenable to Native Mass Spectrometry of Lipoprotein Complexes. J. Am. Soc. Mass Spectrom. 2019, DOI: 10.1007/s13361-019-02174-x.

Native MS of nanodiscs with mixed lipids

New Article in the Journal of the American Chemical Society

Congrats to James, Dane, Guozhi, Ciara, and Deseree on the publication of their paper in JACS! This work demonstrated the first native MS measurement of membrane protein oligomeric state within a lipid bilayer environment. Deseree did an awesome job sketching the concept below. Thanks also to collaborators in the Pemberton and Prell labs. Well done team!

Keener, J.E.Zambrano, D.E.Zhang, G.Zak, C.K.Reid, D.J.; Deodhar, B.S.; Pemberton, J.E.; Prell, J.S.; Marty, M.T. Chemical additives enable native mass spectrometry measurement of membrane protein oligomeric state within intact nanodiscs. J. Am. Chem. Soc. 2019, 141, 1054-1061.

Nanodisc supercharging

National Institutes of Health MIRA Award

Congratulations to Prof. Marty on being awarded the National Institutes of Health/National Institute of General Medical Sciences Maximizing Investigator's Research Award (MIRA) R35 GM128624! This grant will support the lab efforts to employ native mass spectrometry and nanodiscs to understanding membrane protein-lipid interactions.

Congrats to Dane and Jessica on Graduating

Congratulations to Dane Zambrano and Jessica Diesing on their graduation and successful completion of their undergraduate theses. Jessica was also recently awarded the CBC Outstanding Peer Mentor Award and CBC Student of the Month. Congrats!

American Society for Mass Spectrometry Research Award

Congratulations to Prof. Marty on being awarded one of the 2018 ASMS Research Awards! Thanks to Thermo Fisher for sponsoring the award.

New Article in Journal of the American Society for Mass Spectrometry

Check out our new article on MetaUniDec, software for high throughput deconvolution of native mass spectra:

Reid, D. J.; Diesing, J. M.; Miller, M. A.; Perry, S. M.; Wales, J. A.; Montfort, W. R.; Marty, M. T. MetaUniDec: High-Throughput Deconvolution of Native Mass Spectra. J. Am. Soc. Mass. Spectrom. 2018, DOI: 10.1007/s13361-13018-11951-13369.

The software can be downloaded from links in the software tab. Happy deconvolving!

Congrats to Dane Zambrano

Undergrad, Dane Zambrano, has been with the Marty Lab since Day 1. She is graduating soon and has garnered some of the top awards from the CBC department, including the Excellence in Undergraduate Research Award. Congrats to Dane and good luck in grad school at UW Seattle!


New Article in Analytical Chemistry

Check out our new article describing engineering of nanodiscs for native mass spectrometry.

Reid, D.J.; Keener, J.E.; Wheeler, A.P.; Zambrano, D.E.; Diesing, J.M.; Reinhardt-Szyba, M.; Makarov, A.A.; Marty, M.T. "Engineering Nanodisc Scaffold Proteins for Native Mass SpectrometryAnal. Chem. 2017, DOI: 10.1021/acs.analchem.7b03569

Plasmids used in this study can be acquired from Addgene: http://www.addgene.org/Michael_Marty/

Bisgrove Scholar Award

Congratulations to Prof. Marty on being awarded the Bisgrove Scholar Award from Science Foundation Arizona. For more information, take a look at this article.


New Review Article

A review of membrane mimetics for mass spectrometry was recently published in Accounts of Chemical Research. For those interested in learning more about our research, this is a great place to start.

Marty, M.T.; Hoi, K.K.; Robinson, C.V. "Interfacing Membrane Mimetics with Mass Spectrometry" Acc. Chem. Res. 2016, DOI: 10.1021/acs.accounts.6b00379